Animal Species for Developmental Studies: Vertebrates by T. A. Dettlaff (auth.), T. A. Dettlaff, S. G. Vassetzky PDF

By T. A. Dettlaff (auth.), T. A. Dettlaff, S. G. Vassetzky (eds.)

ISBN-10: 0306110326

ISBN-13: 9780306110320

ISBN-10: 1461536545

ISBN-13: 9781461536543

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5-2 g/liter) and with acetonized pituitary powder added (41lg/ml) [65]. It is necessary that the temperature be maintained within the limits of spawning temperatures. Even shortterm cooling or overheating of oocytes covered with a follicle envelope prevents their in vitro maturation under the influence of gonadotropic pituitary hormones [20, 28, 29]. The oocytes should be kept for some time in the solution with pituitary hormones, after which they acquire maturation inertia and are capable of completing their maturation in the Ringer solution without hormones.

Stage 24. Appearance of eye protrusions and thickening of the anterior end of the rudiments of the excretory system (Figs. 18). The suture in the region of fusion of the neural folds is less distinct (24h). Eye protrusions form in the posterior part of the prosencephalon. On each side of the rhombencephalon, thickenings of the epithelium internal layer - ear placodes, rudiments of labyrinths have arisen. In front of and adjoining the brain, a light crescent-shaped plate is delineated, the rudiment of the hatching gland.

12). In the embryo within the envelopes, the tail and a part of the dorsal region as far forward as the pronephros are bent to one side (30d). The embryo can move its tail and head. After the removal of the envelopes it is seen that the tail has begun to straighten (30); it is compressed and surrounded by a narrow fin fold. There is a dense network of blood vessels in the body wall on the dorsal side of the yolk sac (not shown in 30). Stage 31. Tail end reaches heart (Fig. 12). In the embryo within the envelopes, the dorsal region, from the head backward, is bent sideways (31h).

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Animal Species for Developmental Studies: Vertebrates by T. A. Dettlaff (auth.), T. A. Dettlaff, S. G. Vassetzky (eds.)


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