By Curtis A. Williams, Merrill W. Chase
Tools in Immunology and Immunochemistry, quantity IV: Agglutination, supplement, Neutralization, and Inhibition offers details pertinent to direct and oblique agglutination reactions. This ebook covers a number of issues, together with complement-fixation tactics, isolation of supplement elements, hemolytic intermediates, complement-related proteins, and neutralization reactions.
Organized into 3 chapters, this quantity starts off with an summary of test-tube agglutinations which are most well liked for blood grouping with saline agglutinins that require various mins for agglutination. this article then describes blood staff antibodies that agglutinate crimson blood cells suspended in saline. different chapters examine the classical pathway of supplement usage. This booklet discusses besides the complexity of occasions resulting in hemolysis of erythrocytes by way of supplement. the ultimate bankruptcy offers with the power of antitoxin to neutralize diphtheria toxin and explains the quantitative relationships among antigen and antibody.
This ebook is a beneficial source for immunologists, scientists, and learn employees.
Read Online or Download Agglutination, Complement, Neutralization, and Inhibition PDF
Similar immunology books
The time period humanized mouse during this textual content refers to a mouse within which human tissues and cells were transplanted and express an analogous organic functionality as they do within the human physique. that's, the physiological houses and services of tra- planted human tissues and cells could be analyzed within the mouse rather than utilizing a dwelling human physique.
Immunology at a look is designed to be a concise, with ease available creation and revision relief for undergraduate scientific and technology scholars. Following the now conventional, easy-to-use structure, every one subject is gifted as a double-page unfold with key proof followed through clear,informative line diagrams illustrating the basic issues.
This paintings contains elements, half A: thoughts and half B: functions. partially A crucial ideas of pattern guidance, extraction, clean-up, and of tested and potential chromatographic thoughts are mentioned on the subject of mycotoxins. partially B crucial info, scattered within the literature, on thin-layer, liquid, and gasoline chromatography of mycotoxins were compiled.
This e-book facilities on gene treatment and gene move methods to avoid or deal with persistent virus infections. the main target is at the monstrous 3: human immunodeficiency virus (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV). abundant anti-HIV medications are at present to be had within the hospital and the improvement of an efficient mixture treatment has dramatically more advantageous the lifespan and caliber of lifetime of contaminated participants.
- Recent Trends in Antifungal Agents and Antifungal Therapy
- Gene Therapy of Autoimmune Diseases
- Phosphodiesterase Inhibitors
- In Situ Hybridization in Light Microscopy (Methods in Visualization)
Additional resources for Agglutination, Complement, Neutralization, and Inhibition
Immunol. 82, 279 (1959). 2 INDIRECT (PASSIVE) HEMAGGLUTINATION 35 6. S E N S I T I Z A T I O N O F E R Y T H R O C Y T E S i. 2 phos phate-buffered saline t o a final concentration of 2 . 5 % b y volume. 0 ml of distilled water, the lysate is centrifuged clear, a n d hemoglobin is read spectrophotometrically. After this reading, correction of the volume of the " 2 . ,^^ namely, V2 = ( F i O D i ) / O D 2 , where V2 is the adjusted volume to give O D 2 , the optical density desired after the laking procedure; ODi is the optical density read upon laking an aliquot of the original volume 7 i .
C. Boyd and E . Shapleigh, Blood 9, 1195 (1954). P. Speiser, Κ . Baumgarten, and Ο. Kaserer, Ζ. Immunitaetsforsch. I l l , 168 (1954). Μ. Saint-Paul, Transfusion 4, 3 (1961). W. C. Boyd, Vox Sanguinis 8, 1 (1963). K . C. Atwood and S. L . Scheinberg, / . Cell. Comp. Physiol. 52, Suppl. 1, 97 (1958). 6 DIRECT HEMAGGLUTINATION 21 erythrocyte,2^ (i) changing, by chemical alteration, the specificity of lectins (and potentially of antibodies),^^-^^ (j) testing the lattice theory of immune reactions.
4, 456 (1959). a] be carried out a t + 4 ° overnight with a weight of saline equal to 5 to 1 0 times the weight of the ground seed. V a n Loghem*^ recommends for Vicia graminea 8 0 parts of saline, added in three portions, the super n a t a n t suspension being poured off each time before fresh saline is added. Lectin preparations keep well in the frozen state. M o s t of them can be lyophiUzed. T h e specificity of lectins is in some cases greater, in some cases less, than that of human and animal antibodies having similar specificities.
Agglutination, Complement, Neutralization, and Inhibition by Curtis A. Williams, Merrill W. Chase